Loading and Washing Buffer

• 6 M urea
• 0.1  mM  CaCl₂
• 20 mM Tris pH 8.0

Elution Buffer

• 6M urea
• 0.3  M NaCl
• 2 mM EDTA or EGTA
• 20 mM Tris pH 8.0
•  

 handwritten recipe on same page

6 M urea

25mM Tris HCl pH 8

1M NaCl

1        mM EDTA

2        1mM PMSF

• 6 M urea
• 25 mM Tris pH8
• 200mM NaCl
• 3mM CaCl2
• 1mM PMSF