Select Page

Comparative Physiology @ Guelph

Glycerol Relaxing and Skinning Solution

Relaxing Solution
  • 100mM KCl
  • 10mM MOPS
  • 5 mM dipotassium EGTA
  • 9mM MgCl2
  • 4 mM ATP
  • pH 7.0 @ 4.0 C

Dilute 100% volume with glycerol for trabeculae storage solution

Triton X to 1% by volume for skinning solution (first night)