Relaxing Solution

• 100mM KCl
• 10mM MOPS
• 5 mM dipotassium EGTA
• 9mM MgCl₂
• 4 mM ATP
• pH 7.0 @ 4.0 C

Dilute 100% volume with glycerol for trabeculae storage solution

Triton X to 1% by volume for skinning solution (first night)