25mL Poly/Bed 812

20mL DDSA

12.5mL NMA

1.75mL BDMA

4 x 10mL syringes

1 x 5mL syringe

Urine container

Stir bar

Stir plate

• Add Poly/Bed 812, DDSA, and NMA to urine container, each with a different 10mL syringe, while stirring in the fume hood.
• Add BMDA with the 5mL syringe, pushing the plunger rapidly to ensure that all contents are released into the mix.
• Label and store in -20°C freezer for up to 2 months, or at 4°C for 2 weeks.
• Polymerize in 60°C oven for 24 hours.
• Let cool to room temperature before cutting with ultramicrotome.