Select Page

Comparative Physiology @ Guelph

Sterilizing FlexCell Silastic Strips for Cell Culture

  • Sterilize stainless ruler, forceps and razor blade with 70% EtOH, alcohol flame
  • Cut strips (13x34 mm) with razor blade and ruler in sterile hood
  • Transfer strips to sterile petris with sterile forceps
  • Soak strips in 70% EtOH, make sure both sides get wet (1 hr both sides)
  • Aspirate out EtOH in sterile hood
  • Leave in sterile hood for 1 hour with top slightly ajar
  • Flip strips, dry again for 1 hour
  • Arrange 2 strips per 60 mm dish with sterile forceps
  • Place under sterilizing UV light overnight in hood with blower on or sash completely closed