Comparative Physiology @ Guelph
Bug Juices
| 2XYT | 1 Litre |
| Tryptone | 16 Gm |
| Yeast Extract | 10 Gm |
| Sodium Chloride | 5 Gm |
The pH should be about 7. Autoclave.
| LB | 1 Litre |
| Tryptone | 10 Gm |
| Yeast Extract | 5 Gm |
| Sodium Chloride | 10 Gm |
For LB Agar, add 15 Gm Bacto Agar per Liter. Do not use agarose- used for gels, use Bact Agar. The pH should be about 7. Autoclave.
| SOC | 100 Ml |
| Tryptone | 2 Gm |
| Yeast Extract | 0.5 Gm |
| Sodium Chloride | 0.05 Gm |
| Magnesium Chloride | 0.203 Gm |
| Magnesium Sulfate | 0.120 Gm |
| Glucose | 0.360 Gm |
The pH should be about 7. Do not Autoclave, Filter Sterilize only.