Comparative Physiology @ Guelph
Cleaning Phenylsepharose Column
Every cycle, clean with 1 bed volume of 6M urea solution followed by
6-7 bed volumes of column running solution to wash it
opt: Every 5 cycles, clean with 4 bed volumes 0.5-1M NaOH followed by 2 bed volumes of water, followed by 2 bed volumes of buffer