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Comparative Physiology @ Guelph

Cleaning Phenylsepharose Column

Every cycle, clean with 1 bed volume of 6M urea solution followed by

6-7 bed volumes of column running solution to wash it

opt: Every 5 cycles, clean with 4 bed volumes 0.5-1M NaOH followed by 2 bed volumes of water, followed by 2 bed volumes of buffer