TnI Solutions for TnC affinity Column (after CM)

Running Buffer 1 Liter

6 M Urea        360.36g

50mM Tris HCl pH 8 (4^oC)   50 ml 1M

0.2M NaCl  11.68g

3mM CaCl₂  3 ml 1M

1mM PMSF Add just before using

pH to 8.0 at 4C, filter Note: pH at 25^oC is 7.13

Elution Buffer 1 liter

6M Urea  360.36g

50mM Tris HCl pH 8  50ml 1M

1M NaCl  58.44g

2 mM EDTA  4 ml 0.5M EDTA

1mM PMSF

pH to 8.0